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The decarboxylase reactions of members of the Enterobacteriaceae are particularly well studied using lysine decarboxylase broth.
With Taylor's modification, low pH and anaerobic culture requirements are no longer necessary, making the medium relatively simple to regulate.
The bacterium ferments glucose during the early stages of incubation, producing acid as a byproduct and changing the colour to yellow. An alkaline reaction will occur after prolonged incubation if lysine is decarboxylated to cadaverine. The colour then turns purple (a good thing). The reaction is adverse if the colour is still yellow.